Skip to main content
. 2025 Aug 20;55(11):2825–2847. doi: 10.1007/s40279-025-02294-3

Table 2.

A summary of the participants, exercise characteristics, environmental conditions and hydration strategies of the studies included in the heat exposure meta-analysis (in chronological order)

Article Sample size Population Exercise Duration and intensity Outcomes Time point Environmental condition Core, rectal, oesophageal or tympanic temperature Hydration strategy BM change
Fink et al., 1975 [7] 6 Physically active males; 21–39 y Cycling 3*15 min @ 70–85% V˙O2max Glycogen use Exercise-averaged T: 9 °C & 55% RH; H: 41 °C & 15% RH T: + 1.0 °C; H: + 1.8 °C Fluid provided to maintain BM at pre-exercise level T: − 0.2% BM; H: − 0.1% BM
Young et al., 1985 [8] 13 Healthy males; 22 y Cycling 30 min @ 70% V˙O2max RER, glycogen use End of exercise (RER), exercise-averaged (glycogen use) T: 21 °C & 30% RH; H: 49 °C & 20% RH T: 38.0 ± 0.1 °C; H: 38.7 ± 0.1 °C* Ad libitum
Dolny and Lemon, 1988 [44] 8 Males; 28 y Cycling 90 min @ 65% V˙O2max RER Exercise-averaged T: 20 °C & 53% RH; H: 30 °C & 59% RH T: 38.0 ± 0.2 °C; H: 38.2 ± 0.1 °C* Ad libitum
Barnett and Maughan, 1993 [45] 5 Fit males; 26 y Cycling 60 min @ 55% V˙O2max RER, CHOox End of exercise T: 22 °C & 67% RH; H: 35 °C & 60% RH No fluid provided T: − 0.8 ± 0.3 kg; H: − 1.3 ± 0.4 kg*
Snow et al., 1993 [46] 7 Active males; 24 y Cycling 40 min @ 70% V˙O2peak RER Exercise-averaged T: 20 °C & 20% RH; H: 40 °C & 20% RH T: 38.1 ± 0.2 °C; H: 38.7 ± 0.1 °C* No fluid provided
Nielsen and Kroug, 1994a [47] 7 Elite male runners; 27 y Running 60 min @ 75% V˙O2max RER End of exercise T: 18 °C & 50–60% RH; H: 32 °C & 50–60% RH 400 mL of distilled water in both conditions T: − 1.1% BM; H: − 2.2% BM
Nielsen and Kroug, 1994b [47] 5 Endurance trained males; 27 y Cycling 150 min @ 60% V˙O2max RER End of exercise T: 20 °C & 30–40% RH; H: 40 °C & 30–40% RH 400 mL of distilled water in both conditions T: − 0 to − 0.1% BM; H: − 2.5% BM
Febbraio et al., 1994a [6] 13 Endurance trained males; 21 y Cycling 40 min @ 70% V˙O2max (measured in H condition) RER, CHOox, glycogen use End of exercise T: 20 °C & 20% RH; H: 40 °C & 20% RH T: 38.7 ± 0.1 °C; H: 39.7 ± 0.1 °C*
Febbraio et al., 1994b [5] 12 Endurance trained males; 22 y Cycling 40 min @ 70% V˙O2max (measured in H condition) RER, CHOox, glycogen use End of exercise T: 20 °C & 20% RH; H: 40 °C & 20% RH T: 38.6 ± 0.1 °C; H: 39.6 ± 0.1 °C*
Young et al., 1995 [39] 18 Healthy males; 2 groups of 9; 20 y Cycling 60 min @ 60% V˙O2max Glycogen use Post exercise T: 20 °C; H: 35 °C (water immersion)

Group 1 T: 37.4 ± 0.2 °C; H: 38.3 ± 0.1 °C

Group 2 T: 37.9 ± 0.3 °C; H: 38.5 ± 0.1 °C

Hargreaves et al., 1996 [10] 6 Endurance trained males; 20 y Cycling 40 min @ 65% V̇O2peak RER, CHOox End of exercise T: 20 °C & 50% RH; H: 40 °C & 50% RH T: 38.2 ± 0.2 °C; H: 39.1 ± 0.2 °C* T: − 0.7 ± 0.1 kg; H: − 1.2 ± 0.1 kg*
Galloway and Maughan, 1997 [48] 8 Healthy males; 25 y Cycling 60 min @ 70% V˙O2max RER, CHOox End of exercise T: 21 °C & 70% RH; H: 31 °C & 70% RH T: 39.3 °C; H: 39.6 °C* No fluid provided
Cheuvront and Haymes, 2001 [33] 8 Female marathon runners; 37 y Running 30 km @ best marathon race pace RER, CHOox End of exercise T: 20 °C & 54% RH; H: 30 °C & 55% RH T: 38.4 ± 0.1 °C; H: 39.2 ± 0.2 °C Ad libitum T: − 2.4 ± 0.6 kg; H: − 3.2 ± 0.8 kg*
Marino et al., 2001 [49] 9 Highly trained endurance male runners; 25 y Running 30 min @ 70% of peak treadmill running speed RER End of exercise T: 15 °C & 60% RH; H: 35 °C & 60% RH T: 38.6 ± 0.1 °C; H: 38.5 ± 0.2 °C*
Nybo and Nielsen, 2001 [11] 8 Endurance trained males; 23 y Cycling 60 min @ 57% V˙O2max RER, CHOox Exhaustion (30–50 min) T: 18 °C & 40% RH; H: 40 °C & 20% RH T: 37.8 ± 0.1 °C; H: 40.0 ± 0.1 °C* T: 0.3 ± 0.1 L; H: 0.8 ± 0.1 L of pre-warmed water T: − 0.7 ± 0.1% BM; H: − 0.7 ± 0.2% BM
Jentjens et al., 2002 [50] 9 Trained males; 24 y Cycling 90 min @ 55% V˙O2max RER, CHOox, glycogen use Average 60–90 min T: 16 °C & 60% RH; H: 35 °C & 27% RH T: 38.3 ± 0.1 °C; H: 38.9 ± 0.2 °C* Fluid provided every 15 min: 3 mL/kg BM T: − 1.3 ± 0.1 kg; H: − 3.0 ± 0.2 kg*
Yamashita et al., 2005 [51] 6 Healthy males; 19 y Cycling 120 min @ 60% V˙O2max RER End of exercise T: 20 °C & 60% RH; H: 30 °C & 60% RH T: 38.3 ± 0.3 °C; H: 38.9 ± 0.7 °C T: − 1.2 ± 0.2 kg; H: − 2.0 ± 0.9 kg
Hayashi et al., 2006 [12] 13 Healthy males; 25 y Cycling 60 min @ 50% V˙O2peak RER, CHOox End of exercise T: 10 °C; H: 35 °C; H + : 45 °C (perfused suit) T: 37.3 °C; H: 38.6 °C; H + : 39.2 °C* No fluid provided
Hettinga et al., 2007 [52] 10 Well trained males; 24 y Cycling 20 min @ 60% of power output @ V˙O2max RER Exercise-averaged T: 16 °C & 20% RH; H: 36 °C & 3% RH T: 37.0 ± 0.1 °C; H: 37.4 ± 0.6 °C*
Shorten et al., 2009 [53] 11 Active males; 21 y Running 40 min @ 70% V˙O2peak RER, CHOox End of exercise T: 25 °C & 30% RH; H: 36 °C & 30% RH T: 36.1 ± 0.3 °C; H: 38.9 ± 0.3 °C* T: − 0.7 ± 0.2 kg; H: − 0.7 ± 0.4 kg
Fernandez-Elias et al., 2015 [18] 7 Endurance trained males; 25 y Cycling 40 min @ 75% V˙O2max CHOox, glycogen use Exercise-averaged T: 25 °C & 28% RH; H: 36 °C & 25% RH T: 38.5 ± 0.4 °C; H: 39.2 ± 0.4 °C* T: − 2.7 ± 0.3% BM; H: − 3.0 ± 0.2% BM
O'Hearn et al., 2016 [54] 8 Healthy males; 25 y Running 30 min @ 50% V˙O2max CHOox End of exercise T: 23 °C & 35% RH; H: 42 °C & 24% RH T: 37.6 °C; H: 39.0 °C* T: − 0.8% BM; H: − 1% BM*
Collins et al., 2017 [55] 12 Recreationally trained males; 25 y Cycling 60 min @ 60% Wmax CHOox Exercise-averaged T: 20 °C & 60% RH; H: 33 °C & 60% RH T: 37.9 ± 0.3 °C; H: 38 ± 0.3 °C* 500 mL in both conditions
Hoffman et al., 2018 [35] 10 Endurance trained (4 females/6 males); 31 y Running 120 min @ 60% V˙O2max CHOox End of exercise T: 22 °C & 44% RH; H: 30 °C & 35% RH; H + : 35 °C & 26% RH T: 38.4 ± 0.4 °C; H: 38.8 ± 0.6 °C; H + : 39.6 ± 0.7 °C* Ad libitum T: − 1.7 ± 0.7% BM; H: − 1.9 ± 0.7% BM; H + : − 1.7 ± 0.7% BM
Maunder et al., 2020 [56] 20 Endurance trained males; 37 y Cycling 20 min @ VT1 (measured in H condition) CHOox End of exercise T: 18 °C & 60% RH; H: 35 °C & 60% RH Higher in H* Ad libitum
Foster et al., 2023 [37] 10 Trained participants (1 female/9 males); 30 y Cycling 60 min @ 6 W/kg RER, CHOox End of exercise T: 25 °C & 23% RH; H: 40 °C & 22% RH T: 37.9 ± 0.3 °C; H: 38.0 ± 0.3 °C Ad libitum
Schoerberlein et al., 2023 [36] 9 Healthy participants (4 females/5 males); 27 y Cycling 30 min @ 35% (15 min) & 52% (15 min) V˙O2max RER, CHOox End of exercise T: 22 °C & 35% RH; H: 39 °C & 38% RH T: 37.7 ± 0.3 °C; H: 38.1 ± 0.3 °C* T: − 0.5 ± 0.1% BM; H: − 1.6 ± 0.3% BM*
Mora-Rodriguez et al., 2024 [38] 9 Healthy participants (1 females/8 males); 31 y Cycling 50 min @ 58% V˙O2max RER, CHOox, Glycogen use Exercise-averaged T: 21 °C; H: 33 °C T: 36.6 ± 0.6 °C; H: 37.2 ± 0.3 °C* T: − 0.4 ± 0.1 kg; H: − 0.6 ± 0.2 kg*
Rosbrook et al., 2024 [57] 10 Healthy males; 25 y Walking 90 min @ 1.56 m/s with incline to elicit VT1 RER, CHOox End of exercise T: 20 °C & 40% RH; H: 37 °C & 20% RH T: 38.3 ± 0.2 °C; H: 39.3 ± 0.2 °C* T: − 0.9 ± 0.6% BM; H: − 1.7 ± 0.6% BM*

BM body mass, CHOox carbohydrate oxidation, H hot, H+ Second hot condition, RER respiratory exchange ratio, RH relative humidity, T temperate, V˙O2max maximal oxygen consumption, V˙O2peak peak oxygen consumption, VT ventilatory threshold, Wmax maximum workload, y years

*Significant difference was reported between environmental conditions