Figure 3.—
Molecular analysis of tandem duplication and Ercc1X reduction. Allele-specific PCR was performed using either an Ercc1-specific primer (+) or a Ercc1X-specific primer (X) and a reverse primer complementary to sequence outside of the targeting sequence. PCR reactions were run on a standard agarose gel for analysis. The expected 2.4-kb product is seen with the “+” primer in wild type; however, the expected 2.4-kb product is missing with the X primer in Ercc1X and instead a 1.2-kb product is seen. A 800-bp product is seen with the X primer in the duplication, confirming the presence of the 1.6-kb deletion.