Fig. 2. (A–C) Analysis of the receptor specificities of different VEGFs using the Ba/F3 bioassay. Measurement of the viability of Ba/F3 cells expressing the chimeric receptors VEGFR-1/EpoR (A), VEGFR-2/EpoR (B) or VEGFR-3/EpoR (C) in the presence of different VEGFs at the indicated concentrations. Cell viability was determined using the MTT assay. Data represent the mean values from triplicate assays (mean ± SD). (D–G) Biosensor analysis of the interaction of VEGF-C (D and E) and VEGF-C156S (F and G) with VEGFR-3 (D and F) and VEGFR-2 (E and G). Chimeric receptor proteins were immobilized onto a carboxymethylated dextran surface. Growth factors were injected over the surface at a flow rate of 20 µl/min at the indicated concentrations. The sensorgrams shown have been subtracted with the corresponding signal obtained when the same sample was passed over a blank control channel. Kinetic data derived from the biosensor analysis are shown in Table I.