Fig. 7. (A and B) Simultaneous signalling via VEGFR-2 and VEGFR-3 upon VEGF-C stimulation leads to sustained p42/p44 MAPK activation in HMVECs. The p42/p44 MAPK activation was detected by western blotting using phospho-Thr202/Tyr204-MAPK-specific antibodies (A) and CREB phosphorylation using phospho-Ser133-specific antibodies (B). The growth factor concentrations used are: VEGF, 10 ng/ml; VEGF-C, 100 ng/ml; and VEGF-C156S, 500 ng/ml. (C) The VEGFR- 3-induced p42/p44 MAPK activation is mediated via PKC in HMVECs. Effects of inhibition of PKC by GF109203X (GFX), MEK1 by PD98059, and PI-3 kinase by LY294002 on p42/p44 MAPK Thr202/Tyr204 phosphorylation, CREB Ser133 phosphorylation and Akt Ser473 phosphorylation in HMVECs. The growth factor concentrations used are: VEGF, 1 ng/ml; VEGF-C, 10 ng/ml; and VEGF-C156S, 500 ng/ml.