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. Author manuscript; available in PMC: 2006 Nov 1.
Published in final edited form as: Toxicol Sci. 2005 Jun 15;88(1):60–72. doi: 10.1093/toxsci/kfi228

Fig. 6. Saturation binding analysis of X. laevis and mouse AHRs.

Fig. 6

Panels show representative specific binding curves for (A) X. laevis AHR1α, (B) X. laevis AHR1β, and (C) mouse AHR, each synthesized in TnT lysates. Graded concentrations of [3H]TCDD were incubated with TnT reactions diluted in MEDMG buffer as described in Material and Methods. Unbound ligand was removed by extraction with dextran-coated charcoal and total binding quantified by liquid scintillation counting of the supernatant. Nonspecific binding was determined in parallel reactions containing unprogrammed TnT lysates. Curves were fit by nonlinear regression using GraphPad Prism version 4.0b. R2 (goodness of fit) values were 0.884 (AHR1α), 0.924 (AHR1β) and, 0.963 (mouse AHR).