Fig. 10. GPIdeAc^–/– cells produce normal GPI anchors attached to mature mfVSG221. (A–C) Mature mfVSG221 and sVSG221 from wild-type (wt) cells and mfVSG221 from GPIdeAc^–/– (dKO) cells were analysed by positive-ion ES-MS. (D and E) wt and dKO mfVSG221 was exhaustively digested with Pronase and the C-terminal GPI peptide fragments were enriched on a C4 reverse-phase cartridge and analysed on line by negative ion ES-MS. The number of Gal residues attached to the common Ser-EtN-P-Man₃GlcN-PI core is indicated above the m/z values of the [M-2H]^2– ions. Ions indicated with * are lyso-species that have lost one myristic acid.