Fig. 2. Affinity labelling of the inositol deacylase glycoprotein in T.brucei membranes. (A) Bloodstream (Bsf)- and procyclic (Pro)-form T.brucei membranes were incubated with a cocktail of serine esterase/amidase inhibitors, labelled with [³H]DFP, washed, lysed and adsorbed with ConA–agarose. The adsorbed glycoproteins were subjected to SDS–PAGE and fluorography. (B) Bloodstream-form T.brucei membranes were labelled as described above and the glycoproteins were treated with (+) and without (–) PNGase F and subjected to SDS–PAGE and fluorography.