Skip to main content
. 2005 Oct;139(2):779–789. doi: 10.1104/pp.105.068924

Figure 2.

Figure 2.

Effects of TrfA on auxin-dependent degradation of Aux/IAA protein. A and B, Effects of TrfA and MG132 on IAA17/AXR3-GUS fusion protein degradation. Six-day-old HS∷AXR3NT-GUS and HS∷axr3-1NT-GUS transgenic seedlings were heat shocked for 2 h at 37°C to induce the fusion protein expression. After incubation for 20 min at 23°C, the seedlings were incubated in 50 μm TrfA or 50 μm MG132 with/without 1 μm IAA for 60 min (A) or 20 min (B). C, Effect of TrfA on auxin-induced cell elongation mediated by ABP1. The MJ10B line expresses ABP1 under the control of a tetracycline-inducible promoter. The R7 line harbors a corresponding empty vector. Leaf strips from each line were incubated in 5 μm NAA and/or TrfA together with AhTet (inducer) for 12 h after preincubation with AhTet for 4 h. Error bars, se. D, Arabidopsis ATP-dependent proteasome inhibition by TrfA and MG132 in T-87 suspension cells. Error bars, se.