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Effects of TrfA on auxin-enhanced interaction between SCFTIR1 and Aux/IAA. Anti-c-myc immunoblot showing pull-down assays using a synthetic Aux/IAA domain II peptide, and extracts of tir1-1[TIR1myc] plants in the presence or absence of both 0.5 μm IAA and 100 μm TrfA as indicated. The recovery of TIR1myc on domain II peptide beads was assessed by immunoblotting with anti-c-myc antibody.
