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. 2005 Oct;139(2):779–789. doi: 10.1104/pp.105.068924

Figure 4.

Figure 4.

Effect of TrfA on auxin-dependent cell division. A, Inhibition of auxin-induced cell division of auxin-deprived BY2 cells by TrfA. Auxin-starved cells cultured without 2,4-D for 24 h were then incubated with 2,4-D and/or TrfA. Black circle, 1 μm 2,4-D; white circle, without 2,4-D; black square, 1 μm 2,4-D plus 20 μm TrfA; black triangle, 20 μm TrfA. Error bars, se. B, Auxin-starved cells treated with or without 1 μm 2,4-D, and with 10 μm TrfA plus 1 μm 2,4-D for 3 d. Arrow indicates each cell. The photographs are of the same magnification. C, Effects of TrfA on IAA-induced lateral root formation in CycB1;1∷GUS line. Six-day-old seedlings grown in 0.5 μm NPA were treated with 5 μm IAA and/or 50 μm TrfA for 2 d. The expression of CycB1;1 was visualized by histochemical staining. D, Antagonistic effects of TrfA on auxin-induced lateral root formation. Six-day-old seedlings grown in 0.5 μm NPA were treated with various concentrations of IAA and/or TrfA and for 2 d. The number of lateral roots per centimeter of primary root was calculated in each seedling (n = 15). Error bars, se.