Fig. 7. Constitutively activated Rho blocks cell alignment. (A) Cells transiently transfected with empty vector, WT Rho or V14Rho plus GFP were subjected to shear stress or kept under static conditions for 16 h. The cells were fixed, stained with rhodamine–phalloidin and observed by confocal microscopy. The direction of flow is indicated by an arrow. (B) Quantification of cell alignment with the direction of flow. Values are means ± SEM, n = 3; >100 cells were scored per condition.