Table II.
EST characteristics and cluster information
Library
|
ESTs (No.)
|
Average Trimmed Length
|
3′ ESTs Included in TUs (No.)a
|
TUs (No.)b
|
Singletons (No.)c
|
Full Coding Length
|
Inverted Clones
|
||
---|---|---|---|---|---|---|---|---|---|
3′ | 5′ | 3′ | 5′ | ||||||
nt | % | % | |||||||
DG1 | 5,642 | 6,627 | 501 | 528 | 5,149 | 2,858 | 1,938 | 56 | 3.2 |
DSAF1 | 3,057 | 3,497 | 516d | 516d | 2,960 | 1,995 | 1,401 | 14 | 2.3 |
DSBF1 | 2,961 | 785 | 543d | 543d | 2,882 | 1,904 | 1,399 | 18 | 2.0 |
EM1 | 5,126 | 5,405 | 508 | 521 | 4,793 | 2,690 | 1,779 | 52 | 1.6 |
FM1 | 4,976 | 5,336 | 507 | 504 | 4,861 | 2,716 | 1,850 | 50 | 1.2 |
IP1 | 4,936 | 5,067 | 500 | 538 | 4,731 | 2,582 | 1,700 | 49 | 0.5 |
LG1 | 5,015 | 5,316 | 485 | 543 | 4,822 | 2,966 | 2,210 | 52 | 2.6 |
OV1 | 2,578 | 2,810 | 516 | 546 | 2,458 | 1,575 | 1,152 | 53 | 2.9 |
OV2 | 2,615 | 2,787 | 571 | 504 | 2,476 | 1,647 | 1,245 | 52 | 2.3 |
PI1 | 5,077 | 5,203 | 523 | 527 | 4,774 | 2,539 | 1,711 | 53 | 1.4 |
PIC1 | 5,042 | 4,522 | 551 | 592 | 4,823 | 2,646 | 1,699 | 28 | 0.7 |
RHIZ1 | 1,179 | 0 | 426 | n.a.e | 1,087 | 789 | 598 | n.d.f | n.d.f |
RHIZ2 | 5,308 | 5,978 | 510 | 513 | 5,175 | 2,610 | 1,665 | 64 | 1.2 |
WS1 | 5,437 | 5,400 | 537 | 515 | 4,792 | 2,580 | 1,856 | 50 | 3.5 |
Total | 58,949 | 58,733 | 516 | 529 | 55,783 | 16,801 | 7,769g |
Some 3′ ESTs were excluded from the Milestone 3′ EST assembly as explained in “Materials and Methods.”
The total number of TUs represented within the indicated library; because a single TU can have ESTs from many libraries, the sum of the values in this column exceed 16,801.
The number of TUs in which an EST from this library appears only once.
Average is for both 3′ and 5′ sequences together.
Not applicable.
Not determined.
Total number of singletons in the Milestone assembly.