Skip to main content
. 2005 Oct;139(2):896–908. doi: 10.1104/pp.105.065243

Figure 1.

Figure 1.

Molecular analysis of atrad51C T-DNA insertion. A, Genomic organization of the AtRAD51C locus, and the position of the T-DNA insertion at the AtRAD51C locus. Black boxes represent the exons, and white boxes represent 5′ and 3′ untranslated regions. Thick lines indicate probe regions for Southern- and northern-blotting analyses (probe A for Southern blotting and probe B for northern blotting). Arrows indicate PCR primer positions to determine the junction sequences of the T-DNA insertion. B, Southern-blotting analysis of the T-DNA insertion in the AtRAD51C locus. In these experiments, 5 μg of genomic DNA from AtRAD51C+/+ (wild type), AtRAD51C+/− (heterozygous), and atrad51C−/− (homozygous) plants were digested with BamHI or XhoI. The digested DNAs were blotted and probed with the DIG-labeled probe A shown in section A. Asterisks show the nonspecifically hybridized bands because these bands were observed in all lanes regardless of genotypes (+/+, +/−, −/−). C, Northern-blotting analysis of AtRAD51C expression in flower buds of AtRAD51C+/+, and atrad51C−/− plants. In these experiments, 10 μg of total RNAs from flower buds of wild-type and mutant plants were blotted and probed with DIG-labeled probe B shown in section A. RNA Mr markers (0.5–5.0 kb) are shown on the left.