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. 2005 Oct;139(2):896–908. doi: 10.1104/pp.105.065243

Figure 6.

Figure 6.

A, Cisplatin sensitivity of wild-type and atrad51C-cultured cells. Calli were obtained from excised root segments of wild-type and atrad51C plants. These calli were maintained on CIM and subcultured every 3 weeks. Seven-day-old calli were transferred and grown on CIM containing 0 to 50 μm cisplatin. The sensitivity to cisplatin was scored visually 3 weeks later. B, Complementation of atrad51C. Calli from wild-type roots transformed with pZH1 (wild type [pZH1]), calli from the atrad51C roots transformed with pZH1 (atrad51C [pZH1]), and calli from the atrad51C roots transformed with pZHg51C (atrad51C [pZHg51C]) were maintained on CIM containing hygromycin. Sensitivity to cisplatin of transgenic callus was scored as mentioned above. The expression of AtRAD51C mRNA in calli from the atrad51C roots transformed with pZHg51C was similar to that in wild-type callus based on reverse transcription-PCR analysis (data not shown).