Table I.
Effect of A. brassicicola inoculation and MeJA treatment on the expression of AP2/ERF genes
Results obtained in the initial genome-wide RT-Q-PCR screen were verified in a secondary independent time-course expression analysis for each ERF/AP2 gene initially identified as differentially expressed at 6 h after either MeJA treatment or fungal inoculation. Ab, A. brassicicola. Values shown indicate average relative expression ratio to control (data from three independent experiments for both initial screening and secondary time course). Bold text indicates statistically significant induction (P < 0.05).
| Locus No.
|
Name
|
Initial Screening
|
Secondary Time Course
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Ab
|
MeJA
|
Ab
|
MeJA
|
||||||||
| 6 h | 6 h | 1 h | 3 h | 6 h | 24 h | 1 h | 3 h | 6 h | 24 h | ||
| At1g04370 | AtERF14 | 3.5 | 8.3 | 3.3 | 1.0 | 0.41 | 13.9 | 0.7 | 4.7 | 2.7 | 4.4 |
| At1g06160 | 2.7 | 5.7 | 1.0 | 1.2 | 1.3 | 41.8 | 1.1 | 6.3 | 3.2 | 22.6 | |
| At1g28370 | AtERF11 | 2.6 | 2.7 | 1.8 | 2.8 | 4.7 | 8.7 | 1.7 | 6.8 | 3.7 | 3.0 |
| At1g33760 | Tiny-like | 2.7 | 3.0 | 1.6 | 0.86 | 9.8 | 1.5 | 1.6 | 2.2 | 1.1 | 0.9 |
| At2g44840 | AtERF13 | 4.2 | 4.3 | 2.5 | 0.4 | 4.7 | 4.4 | 4.1 | 19.7 | 99.6 | 4.7 |
| At3g15210 | AtERF4 | 2.1 | 2.6 | 1.4 | 1.6 | 1.9 | 2.4 | 2.0 | 2.9 | 2.5 | 3.0 |
| At3g23230 | TDR1 | 14.2 | 2.8 | 1.0 | 1.9 | 30.8 | 5.4 | 1.0 | 1.8 | 0.68 | 3.0 |
| At3g23240 | ERF1 | 4.0 | 5.9 | 1.1 | 2.8 | 1.8 | 11.8 | 6.4 | 4.2 | 13.7 | 24.4 |
| At4g17500 | AtERF1 | 2.3 | 3.7 | 1.3 | 0.79 | 1.0 | 2.5 | 2.4 | 9.1 | 5.6 | 8.8 |
| At5g47220 | AtERF2 | 2.2 | 3.5 | 0.74 | 1.0 | 1.3 | 3.3 | 6.7 | 7.7 | 7.4 | 9.4 |