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. 2005 Oct;139(2):969–978. doi: 10.1104/pp.105.066647

Figure 4.

Figure 4.

Developmental profiles of relative p107 phosphorylation (A and B), and extractable PEPC activity and sensitivity to malate inhibition (C) during COS development. A and B, Clarified COS endosperm extracts were subjected to SDS-PAGE (10% gel; A) or nondenaturing PAGE (7% gel; B) and immunoblotting with anti-(B. napus PEPC)-IgG or anti-APS-IgG (40 μg protein/lane). In-gel PEPC activity staining was also performed following nondenaturing PAGE (B). Inset in A shows a photograph of cross-sections of COS pods at developmental stages III, V, VII, and IX that respectively correspond to the heart-shaped embryo, midcotyledon, full cotyledon, and maturation phases of development (Greenwood and Bewley, 1982). C, Relationship between maximal PEPC activity (measured under optimal assay conditions at pH 8.0) and corresponding I50(malate) (measured at pH 7.3, 0.2 mm PEP) for total PEPC activity in the respective desalted clarified extracts (means ± se of n = 3 separate extracts). PEPC activity of mature COS was too low to allow accurate I50(malate) determinations for this tissue.