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Trm1p-II is N-acetylated. (A) IF for wild-type cells expressing Trm1-II-GST. (B) SDS-PAGE of purified Trm1-II-GST (lanes 1 and 2) and marker proteins (lane 3). (C) N-terminal peptides identified by MS; arrows indicate identified Glu-C cut sites. Bar, 5 μm.
