Figure 6.—

Ice2p is important for the INM location of Trm1-II-GFP but not for the nucleoporin, Nsp1p. (A) Location of Trm1-II-GFP. Trm1-II-GFP is nucleoplasmic in two independent YIL090WΔ/ice2Δ strains and ICE2-GST restores location of Trm1-II-GFP to the INM in the MATa YIL090WΔ/ice2Δ strain. (B) IF for Nsp1p. Nuclear pores are not affected by ice2Δ. Left, IF for Nsp1p showing punctate distribution; right, merge of Nsp1p and DNA locations in the respective strains shows that Nsp1p surrounds the nucleoplasm. Bar, 5 μm.