Fig. 8. Sequence of oligonucleotides used to construct the β-lactamase promoters blaP₁ and blaP₂. The –35 and –10 promoter signals, the start of transcription (+1) and the ribosome-binding site (RBS) are indicated. The sequence of blaP₁ was adapted from Su et al. (1992). In blaP₂, the first base in the –10 region was changed from G to T (arrow), and 5 bp were inserted immediately after the transcription start site to create a SacI site that was used to distinguish easily between the two promoters.