Table II. Three classes of mutant ParAs.
| Class | ParAs | Repressor activity |
Partition |
|
|---|---|---|---|---|
| –ParB | +ParB | activity | ||
| Wild-type | + | ++ | + | |
| I | K122Q | |||
| D251H | ++ | ++ | – | |
| D251N | ||||
| II | K122E | –/+ | –/+ | – |
| D152N | ||||
| III | K122R | –/+ | –/+ | – (ParPD) |
| D152H | ||||
The in vivo properties of the parA alleles (Figure 3 and Table I) divided them into three categories, compared with wild-type parA+: class I are the super-repressors, class II are weak repressors and partition defective, and class III are weak repressors and ParPD. Proteins with the mutations shown in bold were purified for subsequent biochemical analyses.