Fig. 2. The EJC promotes efficient export of spliced mRNAs. (A) Xenopus oocyte nuclei were injected with control RNAs plus the indicated pre-mRNA (lanes 1–18) or unspliced control mRNAs (lanes 19–24). RNA samples from total oocyte (T), nuclear (N) and cytoplasmic (C) fractions were collected immediately (0 min) or 120 min after injection and analyzed by 10% denaturing PAGE. One oocyte equivalent of RNA, from a pool of 10 oocytes, was loaded per lane in all panels. Splicing substrates, products and control RNAs are identified to the left of each gel. (B) Same as (A), with the corresponding Ftz-derived RNAs. Percentages indicate the proportion of each spliced mRNA in the cytoplasm 120 min after injection.