Fig. 3. Kinetics of FhaB maturation. Pulse labelling was performed with BPSM (WT, A), BPGR4 (ΔfhaB, B), BPEC (ΔfhaC, C), BPLC5 (ΔsphB1, D) and BPLC7 (S→A sphB1, E). The cells were pulse-labelled for 20 min and chased for the indicated times. The proteins were precipitated and analysed by SDS–PAGE and autoradiography.