Table I. Deubiquitylating enzymes of S.cerevisiae.

Gene	Mol. wt (kDa)	UbVS labeling	Characterization
Ubp1	93	++	known Ubp
Ubp2	146	++	known Ubp
Ubp3	110	–	known Ubp
Ubp4/Doa4	105	–	known Ubp
Ubp5	92	–	known Ubp
Ubp6	57	++	known Ubp
Ubp7	123	–	putative
Ubp8	54	–	putative
Ubp9	86	–	putative
Ubp10/Dot4	88	–	known Ubp
Ubp11	83	–	known Ubp
Ubp12	143	+	putative
Ubp13	77	ND	putative
Ubp14	91	–	known Ubp
Ubp15	143	++	known Ubp
Ubp16	57	ND	putative
Yuh1	26	++	known Uch

Labeling with [¹²⁵I]UbVS was determined as described in Figure 3; ++ indicates strong labeling, + weak labeling, – no labeling; ND = not determined. The molecular weights of DUBs are based on the predicted sizes listed in the YPD database (http://www.proteome.com/databases/index.html).