Fig. 8. Sorting of Phm5p is blocked by mutation of Lys6, but restored by the addition of ubiquitin. GFP–Phm5p, the K6R mutant, and the mutant with ubiquitin added to the N-terminus of the GFP were expressed in both wild-type cells (WT) and the Δdoa4 mutant. The sequence of the Phm5p cytoplasmic tail is shown at the bottom.