Fig. 7. Partial rescue of the morphological and nuclear phenotype of conditional Dyn2 by Dyn1–Dyn2 fusion proteins. (A) After 24 h in CM-G, cells of strain FB1rDyn2 change their morphology and tend to lose their cell polarity (arrow) while large bud-like extensions are formed (arrowhead). (B) Corresponding DAPI staining of DNA in FB1rDyn2. Note that numerous nuclei accumulate in the deformed mother cell. (C) In FB1rDyn2, nuclei tend to cluster within the cell, suggesting that no nuclear migration occurred. (D) Expression of the fusion protein HDyn1-2BM in strain FB1rDyn2/HDyn1-2BM leads to a partial rescue of morphological phenotype of FB1rDyn2, although cells grew slightly larger. (E and F) Corresponding DAPI staining of DNA revealed that most cells still contain numerous nuclei. However, nuclei are lined up in the presence of HDyn1-2BM and almost no clusters were observed (F). Bars in (A), (B), (D) and (E) correspond to 10 µm, and in (C) and (F) to 3 µm.