Fig. 5. Details of nucleosome–nucleosome interactions in yeast NCP. (A) Stereo view of a section of the |2F_o – F_c| electron density map, calculated at 3.1 Å and contoured at 1σ, showing an Mn²⁺-mediated crystal contact between H2A Glu65 and H2B His52 of one nucleosome (dark red) and H2B′ Glu108 and H2B′ His112 of the neighboring nucleosome (gold). (B) Stereo view of the hydrogen bonds between the H2B C-terminal end of H2B αC (dark red) and residues in histone H3 of a neighboring nucleosome core particle (gold), showing a section of the |2F_o – F_c| electron density map calculated at 3.1 Å and contoured at 8–1σ.