Fig. 4. A mobility shift assay with purified α and a DNA fragment carrying deoP2 [–116 to +25; (A)], a structural part of deoC [+58 to +193; (B)], wild-type malT promoter [–121 to +18; (C)] and a mutant malT promoter [–121 to +18; (D)] (see text for details). The mutant malT promoter carries GCGTCGCGA instead of ATAAAAAAA between –53 and –43 of the wild-type promoter (Tagami and Aiba, 1999). The α concentration in each lane is 0, 30.3, 91, 273.8 and 820 nM from left to right.