Fig. 6. Repression of deoP2 by CytR with RNP carrying intact α (α-WT) (37 nM), α with 256 amino acids (α-256) (150 nM) or α with 235 amino acids (α-235) (150 nM). The CytR concentration used in each lane was as follows: 0 for lanes 1, 5 and 9; 5 nM for lanes 2, 6 and 10; 10 nM for lanes 3, 7 and 11; 20 nM for lanes 4, 8 and 12. (A) The transcription products were resolved on denaturing 8% polyacrylamide gel. (B) deoP2 RNAs were quantified with a β-scanner and the total c.p.m. in the presence of CytR relative to that in the absence of CytR (100%) were plotted as a function of CytR concentration.