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Fig. 1. Cdc42p is associated with the vacuolar membrane. (A) 70 µg of isolated vacuoles (Vac), cytosol (Cyt) or a whole-cell extract (Cell) from strain BJ3505 were analyzed by SDS–PAGE and western blotting for their content of Cdc42p, Ypt7p and Pgk1p. (B) Longer exposure of the blots for Cdc42p and Ypt7p in (A) with the bands visible in the whole-cell extract (lane 3).
