Fig. 1. Overexpression of gro does not affect dpp expression, but brings about repression of Dpp target genes. (A–D) Third instar larval imaginal wing discs, stained for the πMyc or CD2 markers (left) and for β-galactosidase (centre); merge, right. In these, and subsequent figures, anterior is to the left and dorsal up. (A) dpp-lacZ expression (centre, red) is unaffected by groE48 mutant clones, marked by loss of the πMyc marker (left, green), or by gro overexpression (data not shown). In contrast, gro overexpression, in clones marked by loss of CD2 (left, green), leads to the complete repression of omb-lacZ (B) and vgQ-lacZ (C), and to a reduction in brk-lacZ (D) expression levels, in a cell-autonomous manner.