Fig. 5. Nup153-deficient NPCs display increased mobility within the NE. (A) Representative fluorescence recovery after photobleaching (FRAP) series of nuclei assembled in vitro from mock-depleted (M, upper panels) or Nup153-depleted (Δ, lower panels) Xenopus egg extracts, stained with Oregon Orange labelled wheatgerm agglutinin. (B) Quantitation of FRAP in three independent experiments using mock-depleted (open squares) or Nup153-depleted (solid squares) assembled nuclei.