Fig. 6. Effect of wt- or mutant-NK1 on HGF/SF-induced scatter of MDCK colonies. MDCK cells were cultured for 3 days in standard medium before incubation with fresh medium (A), 10^–10 M HGF/SF (B) or 10^–10 M HGF/SF plus the concentrations of wt- or mutant-NK1 indicated in the figure (C–H). After overnight incubation, colonies were photographed using phase-contrast optics. wt-NK1 but not the HP11 or HP12 mutant partially inhibits the activity of full-length HGF/SF.