Fig. 7. Immunofluorescence localization of VASP in WASp105–502-expressing cell line. RBL-2H3 cells expressing FRB–WASp105–502 were plated on to glass coverslips coated with anti-CD25 antibodies and treated with DMSO as control (A and B) or rapamycin (C and D), fixed, and stained with phalloidin (A and C) and anti-VASP antibodies (B and D). Lower magnification fields are shown in the insets. Arrows point at peripheral structures positive for F-actin and VASP, while arrowheads depict internal structures. Confocal sectioning was performed at the ventral cell surface. Bar: 10 µm.