Fig. 8. Accumulation of WASp and VASP in actin-rich phagocytic cups during FcR-mediated phagocytosis. RBL-2H3 cells that express the high affinity IgE receptor (FcεRI) were incubated with IgE-opsonized zymosan particles. After 30 min at 37°C, cells were fixed and labeled with Texas Red–phalloidin to visualize F-actin (A and B), with anti-WASp antibodies (C and D) or antibodies specific for VASP (E and F). Zymosan particles, visualized in the FITC channel, are shown in green in (B), (D) and (F). Confocal optical sections were recorded in the dorsal plane of the cells. Bar, 10 µm.