Fig. 9. Effect of VASP on reconstituted actin-based motility of WASp-coated beads. (A) Extract of BHK-21 cells overexpressing mycFRB–WASp105–502 or control mock-transfected cell extract were incubated with anti-myc tag antibody-coated beads. Unbound proteins (U) or proteins bound to anti-myc beads (B) were subjected to western blot analysis with anti-myc tag antibody. (B–M) Typical phase-contrast images of beads after 90 min of incubation in the reconstituted motility medium. (B) Control bead. (C–F) MycFRB–WASp105–502-coated beads in the motility medium in the absence of VASP. (G–I) Motility medium supplemented with 0.5 µM VASP or (J–M) supplemented with 0.5 µM VASP and 1.6 µM GST–ActAPro241–323. Bar, 10 µm.