Fig. 3. In vitro splicing of PIP7.A (A) and tat (B) in different NES. The extracts are denoted: NE (non-depleted); mockΔNE (mock immunodepleted); oligo(dT)ΔNE [oligo(dT)-chromatography depleted]; and αMC3ΔNE (U2AF65 immunodepleted). U2AF denotes the addition of U2AF activity recovered from the oligo(dT) column. The identities of the splicing products are indicated.