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. 2001 Oct 15;20(20):5748–5758. doi: 10.1093/emboj/20.20.5748

graphic file with name cde564f8.jpg

Fig. 8. Native gel electrophoresis analysing the assembly of spliceosomes on tat pre-mRNA-derived constructs in response to hnRNP A1 (15 ng/µl) and inactivation of the ISS and ESS3 elements. The pre-mRNAs, PIPtat, PIPtat-sA1Δ4 and, as control, PIP7.A, were incubated for 20 min under the indicated conditions before analysing the complexes by native polyacrylamide gel electrophoresis. The positions of complexes H, A, B and C are indicated.