Fig. 5. REF and TAP are involved in viral mRNA export. (A) Oocytes were pre-injected with BSA (lanes 1–4) or ICP27 (lanes 5–12) followed by nuclear injection of RNA mix. The RNA mix was injected alone (lanes 1, 2, 5 and 6), with 14 µM GST–REF (lanes 3, 4, 7 and 8) or with affinity-purified anti-REF antibodies (5 mg/ml) (lanes 11 and 12) or control antibodies (lanes 9 and 10). RNA samples from nuclear (N) and cytoplasmic (C) fractions were collected 1 h after injection. (B) Oocytes were pre-injected with BSA (lanes 3, 4, 7 and 8) or ICP27 (lanes 5, 6, 9 and 10), followed by nuclear injection of RNAs in the presence of 0.1 pmol CTE M36 mutant (lanes 3–6) or 0.1 pmol wild-type CTE RNA (lanes 7–10). RNA samples from nuclear (N) and cytoplasmic (C) fractions were collected immediately (lanes 1 and 2) or 3.5 h after injection (lanes 3–10). In this gel (10%), the lariat and the Ftz mRNA co-migrate.