Fig. 6. ICP27 protein is exported via TAP/NXF1 in the presence of viral mRNA. (A) Labelled ICP27, GST–M10 and Rev proteins were injected into oocyte nuclei alone (lanes 1–6, 15 and 16), in the presence of 0.1 pmol M36 mutant CTE or wt CTE RNA (lanes 7–10) or 5 mg/ml mutant or wt NES conjugate (lanes 11–14). In lanes 15 and 16, the oocytes were pre-incubated in the presence of 200 nM LMB. Oocytes were dissected into nuclear (N) and cytoplasmic (C) fractions immediately (lanes 1 and 2) or after a 3 h incubation (lanes 3–16). (B) Oocytes pre-injected into the cytoplasm with IBB, were injected into the nuclei with labelled ICP27, GST–M10 and Rev proteins alone (lanes 1 and 2), in the presence of 0.1 pmol M36 mutant CTE or wt CTE (lanes 3–6), or 5 mg/ml mutant or wt NES conjugate (lanes 7–10). Oocytes were dissected into nuclear (N) and cytoplasmic (C) fractions after a 3 h incubation.