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. 2025 Oct 15;16:1643445. doi: 10.3389/fendo.2025.1643445

Figure 2.

Histological sections of retinal tissue are shown in panels a, b, and c. Panel a displays three layers labeled GCL, INL, and ONL with darker staining in the GCL. Panel b shows increased staining intensity in the GCL with arrows indicating specific areas of interest. Stars highlight different regions. Panel c has less staining, depicting the same layers with increased clarity in cellular structures.

Rat retinal micropreparations (A – intact retina; B – day 7; C – day 7, negative control). Representative results of VEGF immunohistochemical staining, counterstained with hematoxylin; ×200. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. In (A) minimal VEGF-positive staining in the ganglion cell layer; in (B) white arrows indicate VEGF-positive ganglion cells, white asterisks indicate Müller cells at the periphery of the inner nuclear layer; (C) absence of specific staining.