Table 1.
Primers used for DNA amplification
| Gene amplified | Forward primer (5’–3’) | Reverse primers (5’–3’) |
|---|---|---|
| ABC1K2-CDS | TCTAGAATGAGGAATGCCGTTGTTGCGCTC | TTATTGGCTCTTATTGTGATGGA |
| RT_ABC1K2 (At5g24970) | CTCAACAAGACCATCAATCTTA | CATTCCCGGTGCAAGCTTC |
| GUS | TACACCGACATGTGGAGTGA | CCATACCTGTTCACCGACG |
| pABC1K2.3 | AAGCTTAGAGAACACGTTGCCTGGTG | AGTCCGTTAGATTCACCACTCGGATCC |
| Fw ABC1K2 ATTB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTATGAGGAATGCCGTTGTTG | |
| ABC1K2—stop codon | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTATGAGGAATGCCGTTGTTGCG | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTATTGGCTCTTATTGTG |
| ABC1K2 – no stop codon | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTATGAGGAATGCCGTTGTTGCG | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTGGCTCTTATTGTGATGGAG |
| RFA4 (At2g21420) | AGACTCTTCTGCATTGACTGC | CAAGTTGCTACACTGGACAC |
| EXPA25 (At5g39300) | AATGTAGGAGGCGCTGGAG | CATCACTCGTCGTAACCCTG |
| INVINH1 (At5g46960) | ATATCAGGATATTGTGGAAGACT | CTTCCTTGAAATCATCCTCGC |
| MCM2 (At1g44900) | GGCCAAGGAATACGACATTG | CCAAGTGATCAGCCTTTTTGG |
| HSP (At5g12020 and At5g12030) | CAATCCTCGAAGACATGCTTG | ATCTCATCTCCTTTGATTCCAG |
| MINI3 (At1g55600) | GATGTAGATGAAGATGAAGAGG | TTTGGAGGTGGAGGTTGAGG |
| IKU2 (At3g19700) | TCGCTTGAGATACTTAGA | ATTCCAGAGATTCCAGAA |
| SHB1 (At4g25350) | AAGAATGGTGGAAGACAGAGAT | GAGAAGCAGCAACGATGGTC |
| ACTIN 2 (At3g18780) | GAACTACGAGCTACCTGATG | CTTCCATTCCGATGAGCGAT |
| UBIQUITIN 10 (At4g05320) | AGGACAAGGAAGGTATTCCTC | CTCCTTCTGGATGTTGTAGTC |
The information for the utilisation of each primer is detailed in material and methods