Fig. 6. Increased co-association in vivo of Src and PTPα during mitosis. (A) Immunoprecipitates made with anti-Src antibody from lysates (containing 1.5 mg total cell protein) from unsynchronized (U, odd lanes) or mitotic (M, even lanes) cells overexpressing wt PTPα-HA (lanes 1–2) or PTPα(Y789F)-HA (lanes 3–4) were analyzed by 9% SDS–PAGE and immunoblotted with anti-PTPα or -Src antibody. The positions of molecular weight standards are indicated in kDa. (a) Anti-PTPα immunoblot of anti-Src immunoprecipitate. (b) Anti-Src immunoblot of anti-Src immunoprecipitate. (c) Anti-PTPα immunoblot of cell lysates containing 10 µg total cell protein. (B) Immunoprecipitates made with anti-PTPα antibody from lysates (containing 1.5 mg total cell protein) from unsynchronized or mitotic Src-overexpressor cells were analyzed by 10% SDS–PAGE and immunoblotted with anti-Src or -PTPα antibody. The positions of molecular weight standards are indicated in kDa. (a) Anti-PTPα immunoblot of anti-PTPα immunoprecipitate. (b) Anti-Src immunoblot of anti-PTPα immunoprecipitate. (c) Anti-Src immunoblot of cell lysates containing 5 µg total cell protein.