Table I. Effect of amino acids on [35S]l-cystine accumulation.
Amino acid (10 mM) | % uninhibited± SEM | Greene et al. (1990) |
---|---|---|
l-methionine | 73 ± 12 (n = 4) | 51 ± 4 |
l-leucine | 75 ± 12 (n = 4) | 61 ± 4 |
l-alanine | 69 ± 9 (n = 6) | 86 |
l-valine | 60 ± 7 (n = 6) | 66 |
l-phenylalanine | 87 ± 7 (n = 6) | 78 |
l-proline | 102 ± 9 (n = 6) | 87 |
l-serine | 81 ± 4 (n = 4) | 79 |
l-threonine | 69 ± 7 (n = 6) | 104 |
l-cysteine | 25 ± 4 (n = 7) | n.d. |
l-glutamic acid | 102 ± 11 (n = 4) | n.d. |
Values are expressed as the mean ± SEM of n independent observations, and are compared with those obtained by Greene et al. (1990) for the lysosomal cystine countertransport activity of mouse L-929 fibroblasts.
n.d., not determined.