Fig. 3. Localization of Aut7p and Apg9p–GFP. (A and B) Apg9p–GFP was visualized in Δapg9 cells (CTD1) under growing conditions: (A) Apg9p–GFP, (B) the Nomarski image is overlaid with the fluorescence of Apg9p–GFP. (C and D) Apg9p–GFP in Δapg9Δapg14 cells (GYS29) was identified under growing conditions: (C) Apg9p–GFP, (D) the Nomarski image is overlaid with the fluorescence of Apg9p–GFP. (E–L) Δapg9 cells (CTD1) expressing Apg9p–GFP were treated with rapamycin for 2 h. Immunofluorescence microscopy was performed as described in Materials and methods. (E and I) Apg9p–GFP, (F and J) Aut7p, (G and K) merged images of Apg9p–GFP (green) and Aut7p (red) and (H and L) Nomarski images. Apg9p and Aut7p were occasionally found in close proximity (arrows in K). Bar: 2 µm.