Fig. 5. Localization of Aut7p and the class E compartment. The class E compartment of Δvps4 cells (MBY3) expressing GFP–Aut7p was labelled with FM4-64 as described in Materials and methods, following treatment with rapamycin for 2 h. (A and E) GFP–Aut7p. (B and F) The class E compartments stained with FM4-64 (arrows). (C and G) Merged images of GFP–Aut7p (green) and FM4-64 (red). (D and H) Nomarski images. Bar: 2 µm.