Table I. Yeast strains and plasmids.

Name	Genotype, construction	Source or reference
Yeast strains
ESM356-1	MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200	this study
ESM616	MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SPC42–GFP-KanMX6	this study
ESM1431	MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 BFA1–GFP-His3MX6	this study
ESM1505	MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 Δcdc26::klTRP BFA1–GFP-His3MX6	this study
ESM1508	MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1 spc72-7 BFA1–GFP-His3MX6	this study
ESM1509	MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1::pSM784 Δcdc26::klTRP	this study
ESM1596	MATa ura3-52 leu2Δ1::pSM976 trp1Δ63 his3Δ200 SPC42–RFP-KanMX6	this study
ESM1597	MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SPC42–RFP-KanMX6 SPC110–GFP-klTRP Δkar9::His3MX6	this study
ESM1598	MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SPC42–YFP-His3MX6 pSM1024	this study
ESM1611	MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1::pSM976 Δkar9::klTRP	this study
GPY499	MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 SPC42–RFP-KanMX6 SPC110–GFP-klTRP	this study
GPY572	MATa ura3-52::pCFP-TUB1 leu2Δ1 trp1Δ63 his3Δ200 SPC42–CFP-KanMX6 BFA1–YFP-His3MX6	this study
GPY598	MATa esp1-1 ura3 leu2 lys2 trp1ade2 BFA1–YFP-His3MX6 SPC42::pXHO144	this study
GPY599	MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 BFA1–YFP-His3MX6 SPC42–CFP-KanMX6 Δkar9::klTRP	this study
YPH499	MATa ura3-52 lys2-801 ade2-101 trp1Δ63 his3Δ200 leu2Δ1	Sikorski and Hieter (1989)
Plasmids
p416-GalS	pRS416 containing the GalS promotor	Mumberg et al. (1995)
pCFP-TUB1	pRS306 carrying CFP–TUB1	Jensen et al. (2001)
pRS304	TRP1-based yeast integration vector	Sikorski and Hieter (1989)
pRS305	LEU2-based yeast integration vector	Sikorski and Hieter (1989)
pSM784	pRS305 carrying SPC42–GFP	this study
pSM976	pRS305 containing BFA1–GFP	this study
pSM1024	p416-GalS carrying SPC42–CFP	this study
pXHO144	pRS304 carrying SPC42 (codons 194–363) fused to CFP	He et al. (2000)