Fig. 1. Schematic representation of the T-DNA from pSDM8000. The T-DNA from pSDM8000 was used in co-cultivation experiments to study T-DNA integration by NHR in recombination defective S.cerevisiae strains. The T-DNA contains the KanMX cassette, which consists of the kan resistance gene of the Escherichia coli transposon Tn903 under control of transcriptional and translational sequences of the filamentous fungus Ashbya gossypii TEF gene. This module allows selection of S.cerevisiae transformants resistant against the antibiotic G418 (Wach et al., 1994).