Fig. 2. T-DNA integrates preferentially at (sub)telomeric regions in rad50, mre11 and xrs2 mutants. (A) Schematic representation of a yeast chromosome end, showing the position of the telomeric (G1–3T) repeats, the Y′ and X elements and the centromere. Y′ and X elements are present at only a subset of all chromosomes and are often found in association with internal tracts of (G1–3T) repeats. (B) Junction sequences of T-DNA left end and genomic DNA of the S.cerevisiae rad50, mre11 and xrs2 mutants. Genomic DNA sequences are shown in italics, T-DNA sequences in normal capitals. Bold sequences represent microhomology of the T-DNA left end with the integration site. Filler DNA sequences are underlined and depicted in italics. The numbers above the sequences represent the number of base pairs deleted from the T-DNA left end. Tel. = telomeric region; Subtel. = subtelomeric region; rDNA = ribosomal DNA region; LTR = long terminal repeat of Ty element; – = none of the 16 chromosomes showed an altered mobility; n.d. = not determined; LB′ = remnant of T-DNA left border repeat.