Table II. Genomic distribution of T-DNA integrated by NHR in rad50, mre11, xrs2 and sir4 mutants in comparison with the wild-type after T-DNA transfer from pSDM8000.
| Yeast strain (genotype) | (Sub)telomeric region | LTR | rDNA | Elsewhere |
|---|---|---|---|---|
| rad50Δ | 2 | 0 | 1 | 0 |
| mre11Δ | 4 | 1 | 1 | 0 |
| xrs2Δ | 2 | 0 | 0 | 0 |
| sir4Δ | 0 | 0 | 0 | 6 |
| Wild-typea | 1 | 2 | 1 | 50 |
aPreviously, target sites for T-DNA integration in the genome of S.cerevisiae strain RSY12 were determined in 44 T-DNA transformed lines (Bundock and Hooykaas, 1996; Bundock, 1999). In addition, we determined the position of T-DNA integration in 10 T-DNA transformed S.cerevisiae YPH250 lines.