TABLE 3.
PBM and JAK/STAT signaling in fibroblasts.
| Cells | λ (nm) | Fluence (J/cm2) | Model | Statistical analysis | Outreads | References |
|---|---|---|---|---|---|---|
| WS1 human skin fibroblast cells. Four models, namely, normal (N), wounded (W), diabetic (D) and diabetic wounded (DW) were used | 660 | 5 | In vitro | Experiments: n = 4, assays in duplicate (average used) Software: SigmaPlot 13.0 Tests: One-tailed Student’s t-test, One-Way ANOVA Significance: P < 0.05 Data presentation: Mean ± SEM (with error bars) |
- Irradiated diabetic wounded cells showed a significant increase in EGF (p < 0.001) and activation of its receptor (p-EGFR) - Activation of JAK/STAT pathway (p < 0.001) (p-JAK2, p-STAT1, p-STAT5) was observed and stimulated cell proliferation (p < 0.001), migration (p < 0.05) and viability (p P < 0.001) - PBM at 660 nm and 5 J/cm2 modulated cellular autocrine signaling, particularly the EGF/EGFR loop |
Jere et al. (2018) |
| WS1 human fibroblasts (wounded and diabetic wounded) | 660 | 5 | In vitro | Experiments: n = 3 Analysis: RT2 Profiler PCR Array, ΔΔCT method Normalization: Average of 5 housekeeping genes Fold change: >1 = upregulation, <1 = downregulation (vs controls) Significance: P ≤ 0.05 |
- PBM (600 nm, 5 J/cm2) regulated JAK/STAT pathway genes in both wounded and diabetic wounded WS1 cells - In wounded cells, 2 genes (INSR and SOCS4) were upregulated - In diabetic wounded cells, 21 genes were upregulated, including JAK3, TYK2, and STAT1, STAT2, STAT3, STAT5A, STAT6 - The upregulation of these genes activated JAK/STAT signaling, enhancing wound healing through processes like migration and proliferation |
Jere et al. (2020) |
| Human fibroblast cell culture: wounded (W) and diabetic wounded (DW) | 830 | 5 | In vitro | Experiments: n = 3 Software: Qiagen GeneGlobe Analysis: ΔΔCT method (2^(ΔΔCT)) Normalization: 5 reference genes (ACTB, B2M, GAPDH, HPRT1, RPLP0) Significance: p ≤ 0.05 |
- PBM at 830 nm and 5 J/cm2 altered JAK/STAT pathway genes in both wounded and diabetic wounded cells - In wounded cells, 5 genes (CEBPB, FCGR1A, GATA3, JUN, SOCS3) were upregulated, and 4 downregulated (CSF1R, ISG15, MYC, SMAD2) - In diabetic wounded cells, 6 genes (EGFR, F2, IL2RG, MPL, MYC, OAS1) were downregulated |
Leyane et al. (2024) |